cell culture sk br3 cells Search Results


breast cancer cell line sk br 3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology breast cancer cell line sk br 3
Breast Cancer Cell Line Sk Br 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc01601931-75-5-10?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
breast cancer cell line sk br 3 - by Bioz Stars, 2026-08
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sum159  (CLS Cell Lines Service GmbH)
93
CLS Cell Lines Service GmbH sum159
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Sum159, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc10920906-234-0-11?v=CLS+Cell+Lines+Service+GmbH
Average 93 stars, based on 1 article reviews
sum159 - by Bioz Stars, 2026-08
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sk br 3 cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sk br 3 cells
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Sk Br 3 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc05656026-53-12-29?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
sk br 3 cells - by Bioz Stars, 2026-08
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sl032  (Genecopoeia)
94
Genecopoeia sl032
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Sl032, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/10__1021_slash_cbmi__5c00205-190-13-14?v=Genecopoeia
Average 94 stars, based on 1 article reviews
sl032 - by Bioz Stars, 2026-08
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human breast cancer cell line sk-br-3-luc  (JCRB Cell Bank)
90
JCRB Cell Bank human breast cancer cell line sk-br-3-luc
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Human Breast Cancer Cell Line Sk Br 3 Luc, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pm40128930-50-1-11?v=JCRB+Cell+Bank
Average 90 stars, based on 1 article reviews
human breast cancer cell line sk-br-3-luc - by Bioz Stars, 2026-08
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human bc cell line sk-br-3  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection human bc cell line sk-br-3
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Human Bc Cell Line Sk Br 3, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc11831019-79-1-15?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
human bc cell line sk-br-3 - by Bioz Stars, 2026-08
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sk-br-3  (JCRB Cell Bank)
90
JCRB Cell Bank sk-br-3
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Sk Br 3, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pm36741448-242-0-1?v=JCRB+Cell+Bank
Average 90 stars, based on 1 article reviews
sk-br-3 - by Bioz Stars, 2026-08
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human breast cancer cells expressing firefly luciferase sk-br-3/luc  (JCRB Cell Bank)
90
JCRB Cell Bank human breast cancer cells expressing firefly luciferase sk-br-3/luc
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Human Breast Cancer Cells Expressing Firefly Luciferase Sk Br 3/Luc, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc06972867-202-21-33?v=JCRB+Cell+Bank
Average 90 stars, based on 1 article reviews
human breast cancer cells expressing firefly luciferase sk-br-3/luc - by Bioz Stars, 2026-08
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sk-br-3 cell line  (FUJIFILM)
90
FUJIFILM sk-br-3 cell line
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Sk Br 3 Cell Line, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc09746049__CAS___113___4350___s001-1-1-16?v=FUJIFILM
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sk-br-3 cell line - by Bioz Stars, 2026-08
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erbb2-positive cell-line (sk-br-3) dna  (National Institute of Standards and Technology)
90
National Institute of Standards and Technology erbb2-positive cell-line (sk-br-3) dna
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Erbb2 Positive Cell Line (Sk Br 3) Dna, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pm35379811-257-0-7?v=National+Institute+of+Standards+and+Technology
Average 90 stars, based on 1 article reviews
erbb2-positive cell-line (sk-br-3) dna - by Bioz Stars, 2026-08
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breast cancer cell line sk-br-3  (DS Pharma Biomedical)
90
DS Pharma Biomedical breast cancer cell line sk-br-3
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
Breast Cancer Cell Line Sk Br 3, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+sk+br3+cells/pmc04480363__pone__0130418__s009-1-1-19?v=DS+Pharma+Biomedical
Average 90 stars, based on 1 article reviews
breast cancer cell line sk-br-3 - by Bioz Stars, 2026-08
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sk-br-3-luc, her2-overexpressing breast cancer cells without hormone receptors  (JCRB Cell Bank)
90
JCRB Cell Bank sk-br-3-luc, her2-overexpressing breast cancer cells without hormone receptors
a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and <t>SUM159</t> cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.
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a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and SUM159 cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.

Journal: Communications Biology

Article Title: Repeat DNA methylation is modulated by adherens junction signaling

doi: 10.1038/s42003-024-05990-4

Figure Lengend Snippet: a – c Mean square displacement (MSD) of mVenus spots was measured in sparse and dense MCF10A-BiAD cultures. a Shown are representative trajectories integrated over a lag time of 3 s. N = 20. b Representative violin plot showing the distribution of mVenus spot movement (lag time of 1 s) in data collected from one biological repeat for sparse (blue) and dense (orange) seeded cells. N = 50–140, statistical testing was determined with a two-tailed unpaired t test, **** p < 0.0001. c Quantification of the MSD experiments representatively shown in ( b ). n = 3, N = 50–140. Statistical analysis was done with a two-tailed paired t test, * p < 0.05. d RT-qPCR analysis of α-satellite transcripts showing increased transcription in 5-aza-dC 24 h after drug treatment. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. e RT-qPCR analysis of α-satellite transcripts was performed on RNA isolated from MCF10A cells grown for 24 h in either sparse or confluent conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. f RT-qPCR was used to analyze transcription from α-satellite DNA in MCF10A cells that had been treated for 24 h with the E-cadherin blocking antibody. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ** p < 0.01. g Western blot analysis of cell lysates isolated from MCF10A, SK-BR-3 and SUM159 cells and probed for E-cadherin. GAPDH was used as loading control. h MSRE-qPCR analysis of the α-satellite locus on genomic DNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns not significant. i qPCR analysis of the α-satellite transcripts on RNA isolated from SK-BR-3 and SUM159 cells, which were cultured for 24 h under either sparse or dense conditions. n = 3. Statistical analysis was done with a two-tailed unpaired t test, ns: not significant. j Scheme of newly discovered pathway linking the methylation status and transcription state of satellite DNA to cell density, mediated by E-cadherin. The figure was generated using Biorender. c , d , e , f , h , i In the dot blots, each dot is the mean value obtained for one biological repeat, the line indicates the mean of all biological repeats, and error bars represent their standard deviation. Paired measurements are indicated with the color coding.

Article Snippet: SUM159 (provided by Dr. Thordur Oskarsson, DKFZ) and SK-BR-3 cells (CLS Cell Lines Service GmbH) were cultured in DMEM medium (Gibco) with 10% fetal calf serum (Gibco).

Techniques: Two Tailed Test, Quantitative RT-PCR, Isolation, Blocking Assay, Western Blot, Control, Cell Culture, Methylation, Generated, Standard Deviation